Overview

  • Product nameAnti-PLOD3 antibody
    See all PLOD3 primary antibodies
  • Description
    Mouse polyclonal to PLOD3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PLOD3 protein (Human)

  • Positive control
    • PLOD3 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab67474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Target

  • FunctionForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
  • Involvement in diseaseDefects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
  • Cellular localizationRough endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate 5-dioxygenase 3 antibody
    • bone protein I antibody
    • BP-I antibody
    • LH3 antibody
    • Lysine hydroxylase 3 antibody
    • Lysyl hydroxylase 3 antibody
    • Plod3 antibody
    • PLOD3_HUMAN antibody
    • Procollagen lysine,2 oxoglutarate 5 dioxygenase 3 precursor antibody
    • Procollagen-lysine antibody
    see all

Anti-PLOD3 antibody images

  • All lanes : Anti-PLOD3 antibody (ab67474) at 1/500 dilution

    Lane 1 : PLOD3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa

References for Anti-PLOD3 antibody (ab67474)

ab67474 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"