Overview

  • Product nameAnti-PLOD3 antibody
    See all PLOD3 primary antibodies
  • Description
    Mouse polyclonal to PLOD3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PLOD3 (NP_001075.1)

  • Positive control
    • HepG2 cell lysate, PLOD3 transfected 293T cell lysate and HeLa cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab89263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
    • Involvement in diseaseDefects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency) [MIM:612394]; also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome.
    • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
    • Cellular localizationRough endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2-oxoglutarate 5-dioxygenase 3 antibody
      • bone protein I antibody
      • BP-I antibody
      • LH3 antibody
      • Lysine hydroxylase 3 antibody
      • Lysyl hydroxylase 3 antibody
      • Plod3 antibody
      • PLOD3_HUMAN antibody
      • Procollagen lysine,2 oxoglutarate 5 dioxygenase 3 precursor antibody
      • Procollagen-lysine antibody
      see all

    Anti-PLOD3 antibody images

    • Anti-PLOD3 antibody (ab89263) at 5 µg/ml + HepG2 cel lysate at 50 µg

      Predicted band size : 85 kDa
    • All lanes : Anti-PLOD3 antibody (ab89263) at 5 µg/ml

      Lane 1 : PLOD3 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 85 kDa
    • ab89263 at 10µg/ml staining PLOD3 on HeLa cell by immunofluorescence

    References for Anti-PLOD3 antibody (ab89263)

    ab89263 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab89263.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"