Overview

  • Product nameAnti-PLS1 antibody
    See all PLS1 primary antibodies
  • Description
    Mouse monoclonal to PLS1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Partial recombinant human PLS1 protein (NP_002661: amino acids 1 to 103) with a 26 kDa tag

  • Positive control
    • Recombinant PLS1 protein; Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.1 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for the full length protein: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePlastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. Fimbrin is a 68-kD actin-bundling protein.
    • Cellular localizationCytoplasm
    • Database links
    • Alternative names
      • Fimbrin antibody
      • I Fimbrin antibody
      • I Plastin antibody
      • Intestine specific plastin antibody
      • Plastin 1 (I isoform) antibody
      • Plastin 1 antibody
      • Plastin1 antibody
      see all

    Anti-PLS1 antibody images

    • Anti-PLS1 antibody (ab76510) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 70 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-PLS1 antibody (ab76510) at 5 µg/ml + Jurkat cell lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 70 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Application of ab76510 in Elisa.
      Detection limit for recombinant, tagged PLS1 is approximately 3ng/ml as a capture antibody.

    References for Anti-PLS1 antibody (ab76510)

    ab76510 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76510.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"