PLTP Activity Fluorometric Assay Kit (ab65386)

Overview

  • Product namePLTP Activity Fluorometric Assay Kit
    See all Plasma phospholipid transfer protein kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Rabbit, Human
  • Product overview

    Abcam's PLTP Activity Fluorometric Assay Kit uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).
    Visit our FAQs page for tips and troubleshooting.

     

    This product is being discontinued. We have created an improved version of this product: PLTP Activity Assay Kit II (Fluorometric) (ab196998).

  • Notes

    Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevancePlasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance.
  • Cellular localizationSecreted.
  • Alternative names
    • HDLCQ9
    • Lipid transfer protein II
    • Phospholipid transfer protein
    • PLTP
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab65386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

PLTP Activity Fluorometric Assay Kit images

  • Plasma Phospholipid Transfer Protein Activity Assay using ab65386.

Protocols

References for PLTP Activity Fluorometric Assay Kit (ab65386)

ab65386 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for your interest in Abcam products.

1) Yes, please use black plates for all fluorometric assays.
2) Yes, you should make a master mix with all components except the samples and distribute it across the well...

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Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

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I am sorry to confirm that this particular kit is sourced externally. As discussed on the phone, I have nowconfirmed that theinformation on the donor and acceptor molecules provided in ab65386 ...

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Thank you for your inquiry. The 10X assay buffer should NOT be diluted before combining it with the other components of the reaction mixture: 10 µl Donor Molecule 10 µl Acceptor Molecule 20 µl 10X Plasma Phospholipid Transfer Protein Assay Bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"