Overview

  • Product name(±)-Blebbistatin
  • Description
    Potent, selective, inhibitor of myosin II
  • Biological descriptionCell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and in vitro motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120425 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    (±)-Blebbistatin images

    • ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for (±)-Blebbistatin (ab120425)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall



    It sounds like there is some precipitation because the product hasn’t equilibrated to room temperature fully before use. Precipitation can occur easily when thawing frozen DMSO solutions and the aliquots need to be inspected carefully b...

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    Thank you for your enquiry and your interest in our products.

    I can confirm that this product (ab120425: Blebbistatin) is in solid form and we sell itin two different unit sizes i.e. 5 and 25 mg.

    I hope this helps and if I can assis...

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