Anti-PLVAP antibody [174/2] (ab81719)

Overview

  • Product nameAnti-PLVAP antibody [174/2]
    See all PLVAP primary antibodies
  • Description
    Mouse monoclonal [174/2] to PLVAP
  • Tested applicationsWB, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Isolated hilar vessels from human lymph nodes

  • Positive control
    • Human tonsil cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab81719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Typical starting dilution 1/50.
    ICC/IF: Typical starting dilution 1/50.
    IHC-Fr: Typical starting dilution 1/50.
    WB: Typical starting dilution 1/50. Predicted molecular weight: 50 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
    • Tissue specificityExpressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
    • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • FELS antibody
      • Fenestrated endothelial linked structure protein antibody
      • Fenestrated endothelial-linked structure protein antibody
      • gp68 antibody
      • Hypothetical protein DKFZp667O074 antibody
      • Meca 32 antigen antibody
      • MECA32 antigen antibody
      • Panendothelial cell antigen antibody
      • Plasmalemma vesicle associated protein antibody
      • Plasmalemma vesicle protein 1 antibody
      • Plasmalemma vesicle-associated protein antibody
      • Plvap antibody
      • PLVAP_HUMAN antibody
      • PV 1 antibody
      • PV-1 antibody
      • PV1 antibody
      • PV1 protein antibody
      see all

    Anti-PLVAP antibody [174/2] images

    • ab81719 at 1/50 dilution staining PLVAP in human tonsil by Immunofluorescence.
    • Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-PLVAP antibody [174/2] (ab81719)

    ab81719 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval
    Sample Cynomolgus Monkey Tissue sections (bone marrow)
    Specification bone marrow
    Permeabilization No
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted Nov 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"