Overview

  • Product nameAnti-PLVAP antibody [MECA-32]
    See all PLVAP primary antibodies
  • Description
    Rat monoclonal [MECA-32] to PLVAP
  • SpecificityThis antibody is specific to PLVAP.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Mouse lymph node stromal cells.

  • Positive control
    • Embryonic and adult mouse endothelial cell surfaces with the exceptions of cardiac, skeletal muscle and brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab27853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed sections.

ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 50 - 55 kDa (predicted molecular weight: 51 kDa).

Target

  • FunctionInvolved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
  • Tissue specificityExpressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
  • Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • FELS antibody
    • Fenestrated endothelial linked structure protein antibody
    • Fenestrated endothelial-linked structure protein antibody
    • gp68 antibody
    • Hypothetical protein DKFZp667O074 antibody
    • Meca 32 antigen antibody
    • MECA32 antigen antibody
    • Panendothelial cell antigen antibody
    • Plasmalemma vesicle associated protein antibody
    • Plasmalemma vesicle protein 1 antibody
    • Plasmalemma vesicle-associated protein antibody
    • Plvap antibody
    • PLVAP_HUMAN antibody
    • PV 1 antibody
    • PV-1 antibody
    • PV1 antibody
    • PV1 protein antibody
    see all

References for Anti-PLVAP antibody [MECA-32] (ab27853)

This product has been referenced in:
  • Wang XL  et al. VEGFR-3 blocking deteriorates inflammation with impaired lymphatic function and different changes in lymphatic vessels in acute and chronic colitis. Am J Transl Res 8:827-41 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27158372) »
  • Menezes MJ  et al. The extracellular matrix protein laminin a2 regulates the maturation and function of the blood-brain barrier. J Neurosci 34:15260-80 (2014). Read more (PubMed: 25392494) »

See all 4 Publications for this product

Product Wall

Thank you for your patience.

For ab27853, it is as I had thought in my last email.The application was added based on publications showing that [MECA-32] has worked in IHC-Fr, e.g.

"The Adnectin CT-322 is a novel VEGF receptor ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the product ab2350 did not perform as stated on the datasheet and for the inconvenienc...

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Thank you for contacting us. While I am unaware of any references describingACVRL1 expression in tumors, the human protein atlas (linked below) does show that "distinct cytoplasmic staining with a granular pattern was observed in thyroid cancers, ...

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