Tissue/ cell preparation: Mouse lymph node stromal cells.
Our Abpromise guarantee covers the use of ab27853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
Acetone fixed sections.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 50 - 55 kDa (predicted molecular weight: 51 kDa).|