• Product nameAnti-Plzf antibody [5B3]
    See all Plzf primary antibodies
  • Description
    Mouse monoclonal [5B3] to Plzf
  • Tested applicationsELISA, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human Plzf expressed in E. Coli.

  • Positive control
    • Hela cell lysate. Hela cells



Our Abpromise guarantee covers the use of ab104854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Information by UniProt
  • Database links
  • Alternative names
    • AI467657 antibody
    • lu antibody
    • PLZF antibody
    • Promyelocytic leukemia zinc finger protein antibody
    • ZBT16_HUMAN antibody
    • ZBTB16 antibody
    • Zfp145 antibody
    • Zinc finger and BTB domain containing 16 antibody
    • zinc finger and BTB domain containing protein 16 antibody
    • Zinc finger and BTB domain-containing protein 16 antibody
    • Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody
    • Zinc finger protein 145 antibody
    • Zinc finger protein PLZF antibody
    • Znf145 antibody
    see all

Anti-Plzf antibody [5B3] images

  • Anti-Plzf antibody [5B3] (ab104854) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 74 kDa
  • Immunofluorescence analysis of Hela cells using ab104854 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Overlay histogram showing K562 cells stained with ab104854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Plzf antibody [5B3] (ab104854)

This product has been referenced in:
  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Marmoset (common) . Read more (PubMed: 25624265) »
  • Schwab KR  et al. Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes. Dev Biol 373:64-71 (2013). Read more (PubMed: 23063797) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde

Zachary Yu-Ching Lin

Verified customer

Submitted Jun 05 2013