Anti-Plzf antibody - N-terminal (ab189849)

Overview

  • Product name
    Anti-Plzf antibody - N-terminal
    See all Plzf primary antibodies
  • Description
    Rabbit polyclonal to Plzf - N-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Plzf aa 1-220 (N terminal).
    Sequence:

    MDLTKMGMIQ LQNPSHPTGL LCKANQMRLA GTLCDVVIMV DSQEFHAHRT VLACTSKMFE ILFHRNSQHY TLDFLSPKTF QQILEYAYTA TLQAKAEDLD DLLYAAEILE IEYLEEQCLK MLETIQASDD NDTEATMADG GAEEEEDRKA RYLKNIFISK HSSEESGYAS VAGQSLPGPM VDQSPSVSTS FGLSAMSPTK AAVDSLMTIG QSLLQGTLQP


    Database link: Q05516

  • Positive control
    • HeLa, THP1, mouse lung, mouse heart, mouse brain extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab189849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 74 kDa.

Target

  • Function
    Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Skeletal defects, genital hypoplasia, and mental retardation
    A chromosomal aberration involving ZBTB16 may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with RARA.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 9 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI467657 antibody
    • lu antibody
    • PLZF antibody
    • Promyelocytic leukemia zinc finger protein antibody
    • ZBT16_HUMAN antibody
    • ZBTB16 antibody
    • Zfp145 antibody
    • Zinc finger and BTB domain containing 16 antibody
    • zinc finger and BTB domain containing protein 16 antibody
    • Zinc finger and BTB domain-containing protein 16 antibody
    • Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia) antibody
    • Zinc finger protein 145 antibody
    • Zinc finger protein PLZF antibody
    • Znf145 antibody
    see all

Images

  • All lanes : Anti-Plzf antibody - N-terminal (ab189849) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : THP1 cell extract
    Lane 3 : Mouse lung extract
    Lane 4 : Mouse heart extract
    Lane 5 : Mouse brain extract


    Predicted band size : 74 kDa

References

ab189849 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Mar 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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