Synthetic peptide within Human PM20D2 aa 403-433 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8IYS1
Our Abpromise guarantee covers the use of ab171142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 48 kDa.|
Confocal immunofluorescent analysis of Hela cells labeling PM20D2 with ab171142 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling PM20D2 with ab171142 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab171142 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"