Anti-PM20D2 antibody - C-terminal (ab171142)

Overview

  • Product name
    Anti-PM20D2 antibody - C-terminal
  • Description
    Rabbit polyclonal to PM20D2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PM20D2 aa 403-433 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8IYS1

  • Positive control
    • Human placenta tissue; K562 cell line lysate: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171142 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 48 kDa.

Target

  • Sequence similarities
    Belongs to the peptidase M20A family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACY1L2 antibody
    • Aminoacylase 1 like 2 antibody
    • Aminoacylase 1 like protein 2 antibody
    • Aminoacylase-1-like protein 2 antibody
    • bA63L7.3 antibody
    • P20D2_HUMAN antibody
    • Peptidase M20 domain containing 2 antibody
    • Peptidase M20 domain-containing protein 2 antibody
    • Pm20d2 antibody
    see all

Images

  • Confocal immunofluorescent analysis of Hela cells labeling PM20D2 with ab171142 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-PM20D2 antibody - C-terminal (ab171142) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 48 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling PM20D2 with ab171142 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171142 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171142.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up