Anti-Pma1 antibody [40B7] (ab4645)
Key features and details
- Mouse monoclonal [40B7] to Pma1
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Pma1 antibody [40B7] -
Description
Mouse monoclonal [40B7] to Pma1 -
Host species
Mouse -
Specificity
Reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Tissue, cells or virus corresponding to Pma1. Yeast nuclear prep
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Sterile filtered. -
Clonality
Monoclonal -
Clone number
40B7 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
(cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. (cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots. |
Target
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Relevance
Pma1p is a plasma membrane ATPase. -
Database links
- Entrez Gene: 852876 Saccharomyces cerevisiae
- SwissProt: P05030 Saccharomyces cerevisiae
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Alternative names
- Plasma membrane ATPase 1 antibody
- Plasma membrane H+ ATPase antibody
- Pma 1 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (28)
ab4645 has been referenced in 28 publications.
- Gersing SK et al. Mapping the degradation pathway of a disease-linked aspartoacylase variant. PLoS Genet 17:e1009539 (2021). PubMed: 33914734
- Santos-Pereira C et al. Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae. Int J Biol Macromol 171:343-357 (2021). PubMed: 33421469
- Nightingale DJH et al. A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae. Bio Protoc 9:e3303 (2019). PubMed: 33654815
- Tremblay-Laganière C et al. Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function. Environ Sci Pollut Res Int 26:2375-2386 (2019). PubMed: 30467749
- Locke MN & Thorner J Rab5 GTPases are required for optimal TORC2 function. J Cell Biol 218:961-976 (2019). PubMed: 30578283