Overview

  • Product nameAnti-PMCA1 antibodySee all PMCA1 primary antibodies ...
  • Description
    Rabbit polyclonal to PMCA1
  • SpecificityDetects PMCA 1a and 1b ATPase.
  • Tested applicationsICC/IF, ELISA, ICC, IP, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide corresponding to Rat PMCA1 aa 5-22.
    Sequence:

    ANNSVAYSGVKNSIKEAN

Properties

Applications

Our Abpromise guarantee covers the use of ab3528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300. PubMed: 16803870
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml.
WB 1/1000. This antibody detects ~134 kDa and ~130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.
IHC-Fr 1/20.
IHC-P 1/20.

Target

  • FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • Tissue specificityIsoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • DomainThe calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT2B1_HUMAN antibody
    • ATP2B1 antibody
    • ATPase Ca++ transporting plasma membrane 1 antibody
    • Plasma membrane calcium ATPase 1 antibody
    • Plasma membrane calcium ATPase isoform 1 antibody
    • Plasma membrane calcium pump isoform 1 antibody
    • Plasma membrane calcium transporting ATPase 1 antibody
    • Plasma membrane calcium-transporting ATPase 1 antibody
    • PMCA 1 antibody
    • PMCA1 antibody
    see all

Anti-PMCA1 antibody images

  • ab3528 at 1/100 staining rat brain hippocacmpal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubation with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview


  • developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 123 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr R Empson

    See Abreview

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase ab3528 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-PMCA1 antibody (ab3528)

This product has been referenced in:
  • Kosiorek M  et al. Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: Implications for catecholamine secretion by PC12 cells. Biochem Biophys Res Commun 411:235-40 (2011). WB ; Rat . Read more (PubMed: 21740891) »
  • Mohamed TM  et al. Plasma membrane calcium pump (PMCA4)/neuronal nitric oxide synthase complex regulates cardiac contractility through modulation of a compartmentalized cyclic nucleotide microdomain. J Biol Chem : (2011). WB ; Mouse . Read more (PubMed: 21965681) »

See all 7 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (neonatal rat cardiomyocytes)
Loading amount 50 µg
Specification neonatal rat cardiomyocytes
Gel Running Conditions Reduced Denaturing (%8 tris HCL)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat hippocampal slice and slice culture)
Loading amount 25 µg
Specification Rat hippocampal slice and slice culture
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. R Empson

Verified customer

Submitted Aug 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Brain Hippocampal Slices and Slice Cultures)
Specification Rat Brain Hippocampal Slices and Slice Cultures
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 1% Triton X-100
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. R Empson

Verified customer

Submitted Aug 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"