Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PML+RARa Fusion. This peptide comprises the region across the PML-RAR alpha junction in the L-type fusion protein.
(Peptide available as ab46898.)
Image courtesy of Christian Marx (University of Jena, Germany)
Anti-PML+ RARα Fusion antibody (ab43152) reactivity with reduced NB4 (A and B) and K562 (B) cell lysate. After SDS-PAGE (where 100μg lysate was loaded in A and 20μg lysate in B), membranes were blocked in 5% Milk in PBS + 0.1% Tween for 1h at 25°C before incubation with ab43152 (1:5,000 dilution in 1% Milk PBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.