Overview

  • Product nameAnti-PMM1 antibody
    See all PMM1 primary antibodies
  • Description
    Rabbit polyclonal to PMM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (AVTAQAARRK ERVLCLFDVD GTLTPARQKI DPEVAAFLQK LRSRVQIGVV) of Human PMM1 (NP_002667).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.
  • Tissue specificityStrong expression in liver, heart, brain, and pancreas; lower expression in skeletal muscle.
  • PathwayNucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.
  • Sequence similaritiesBelongs to the eukaryotic PMM family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain glucose-1,6-bisphosphatase antibody
    • Phosphomannomutase 1 antibody
    • PMM 1 antibody
    • pmm1 antibody
    • PMM1_HUMAN antibody
    • PMMH 22 antibody
    • PMMH-22 antibody
    • PMMH22 antibody
    • Sec53 antibody
    see all

Anti-PMM1 antibody images

  • Anti-PMM1 antibody (ab94606) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Gel concentration: 12%
  • Anti-PMM1 antibody (ab94606) at 1/1000 dilution + Human fibroblasts whole cell lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-rabbit at 1/2500 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 30 kDa


    Exposure time : 10 minutes

    See Abreview

References for Anti-PMM1 antibody (ab94606)

ab94606 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (whole embryo lysate)
Loading amount 50 µg
Specification whole embryo lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblasts)
Loading amount 50 µg
Specification Human fibroblasts
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"