PMN Elastase Human ELISA Kit (ab119553)

Overview

  • Product namePMN Elastase Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Overall 4.8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    1.98 pg/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 104 96% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.

    PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • Tissue specificityBone marrow cells.
  • Involvement in diseaseDefects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names
    • Bone marrow serine protease
    • ELANE
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • Elastase-2
    • ELNE_HUMAN
    • GE
    • granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • Neutrophil elastase
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

PMN Elastase Human ELISA Kit images

  • Representative Standard Curve using ab119553.

Protocols

References for PMN Elastase Human ELISA Kit (ab119553)

ab119553 has not yet been referenced specifically in any publications.

Product Wall

The PMN elastase ELISA ab119553 has not, to our knowledge, been used to test sputum samples, but it will in theory be compatible with that sample type. The question is whether the assay will be sensitive enough to detect the amounts of elastase present...

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Elastase levels in gut mucosa lysates

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the kit's protocol from start to finish.
The samples were whole tissue lysates of human gut mucosa which were assayed in various dilutions (in PBS). Have not tried any other sample type.
Kit is very easy to use and works just like any standard ELISA.
Got consitently good results. Will buy it again.
Username

Abcam user community

Verified customer

Submitted Mar 13 2014



This kit can be used with serum and plasma.


This kit works with plasma treated with EDTA or citrate.
We have no information pertaining to the use of other anti-coagulants in use with this kit.

I am sorry for the delay in getting back to you.

I now have further information to share with you in regards to the Neutrophil Elastase ELISA you are hoping to set up.

If you would be interested in setting up your own ELISA I have loo...

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Thank you for contacting us and your interest in our products.

I am sorry but having looked into it, we are unfortunately not able to provide the standard or high/low samples for the PMN Elastase Human ELISA kit (ab119553) separately.

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Thank you for contacting us.
Unfortunately, it is not possible to obtain the standard and detection antibody separately for this kit. Also, the coating antibody is bound on the plates and is not available separately either.
I am sorry to not ...

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Thank you for contacting Abcam regarding ab119553.


I can confirm that this kit will only recognize the complex of PMN elastase/alpha 1-PI complex standards and also our standard is lyophilized complex.


I hope this inform...

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Ihave issued a freekit, 8-hydroxy 2 deoxyguanosine ELISA Kit, ab101245,with the order number 1070428.

To check the status of the order please contact our Customer Service team and reference this number.

Again, I apologize for any ...

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Thanks for your email.

As we discussed yesterday, I am happy to provide one kit of your choice free of charge. We currently have 8-hydroxy 2 deoxyguanosine ELISA Kit (ab101245) in stock.. If you'd like I will send one out to you right away.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"