Overview

  • Product nameAnti-PMP22 antibody
    See all PMP22 primary antibodies
  • Description
    Rabbit polyclonal to PMP22
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Human)

  • Positive control
    • IHC-P: Human pancreas tissue. ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/250 - 1/4000. 1/250 (direct immunofluorescent detection) - 1/4000 (ABC/DAB detection).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMight be involved in growth regulation, and in myelinization in the peripheral nervous system.
  • Involvement in diseaseDefects in PMP22 are the cause of Charcot-Marie-Tooth disease type 1A (CMT1A) [MIM:118220]; also known as hereditary motor and sensory neuropathy IA. CMT1A is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1A inheritance is autosomal dominant.
    Defects in PMP22 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    Defects in PMP22 are a cause of hereditary neuropathy with liability to pressure palsies (HNPP) [MIM:162500]; an autosomal dominant disorder characterized by transient episodes of decreased perception or peripheral nerve palsies after slight traction, compression or minor traumas.
    Defects in PMP22 are the cause of Charcot-Marie-Tooth disease type 1E (CMT1E) [MIM:118300]; also known as Charcot-Marie-Tooth disease and deafness autosomal dominant. CMT1E is an autosomal dominant form of Charcot-Marie-Tooth disease characterized by the association of sensorineural hearing loss with peripheral demyelinating neuropathy.
    Defects in PMP22 may be a cause of inflammatory demyelinating polyneuropathy (IDP) [MIM:139393]. IDP is a putative autoimmune disorder presenting in an acute (AIDP) or chronic form (CIDP). The acute form is also known as Guillain-Barre syndrome.
  • Sequence similaritiesBelongs to the PMP-22/EMP/MP20 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMT1A antibody
    • CMT1E antibody
    • DSS antibody
    • GAS-3 antibody
    • GAS3 antibody
    • Growth Arrest Specific 3 antibody
    • Growth arrest-specific protein 3 antibody
    • HMSNIA antibody
    • HNPP antibody
    • MGC20769 antibody
    • Peripheral myelin protein 22 antibody
    • PMP-22 antibody
    • PMP22 antibody
    • PMP22_HUMAN antibody
    • Sp110 antibody
    • Trembler antibody
    see all

Anti-PMP22 antibody images

  • ab155006 stained SKNSH cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15506 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Immunostaining of PMP22 in human brain (multiple sclerosis sample) using ab15506 (1:200). Secondary antibody: Goat anti rabbit Alexa488.
  • Immunostaining of PMP22 in human spinal cord (multiple sclerosis sample) using ab15506 (1:2000) and chromogenic detection with ABC amplification.
  • ab15506 staining PMP22 in human pancreas by Immunohistochemistry (FFPE-sections).

References for Anti-PMP22 antibody (ab15506)

This product has been referenced in:
  • Patzig J  et al. Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci. J Neurosci 31:16369-86 (2011). Read more (PubMed: 22072688) »
  • Koeppen AH  et al. The dorsal root ganglion in Friedreich's ataxia. Acta Neuropathol 118:763-76 (2009). IHC-P ; Human . Read more (PubMed: 19727777) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Rat Cell (oligodendrocytes)
Specification oligodendrocytes
Permeabilization Yes - saponine
Fixative acetic acide 5% - ethanol 95%
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Submitted Mar 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"