Overview

  • Product name
  • Description
    Goat polyclonal to PMP70
  • Specificity
    ab81904 is expected to recognize one isoform (NP_002849.1) only.
  • Tested applications
    Suitable for: WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide:

    C-PDGREDQKRKGISD

    , corresponding to internal sequence amino acids 526-539 of Human PMP70 (NP_002849.1).

  • Positive control
    • Human Kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
ELISA 1/16000. Peptide ELISA
ICC Use a concentration of 2.5 - 3.8 µg/ml.

Target

  • Function
    Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • Cellular localization
    Peroxisome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa peroxisomal membrane protein antibody
    • ABC 43 antibody
    • ABC D3 antibody
    • ABC43 antibody
    • ABCD 3 antibody
    • Abcd3 antibody
    • ABCD3 protein antibody
    • ABCD3_HUMAN antibody
    • ATP binding cassette sub family D (ALD) member 3 antibody
    • ATP binding cassette sub family D member 3 antibody
    • ATP-binding cassette sub-family D member 3 antibody
    • CBAS5 antibody
    • dJ824O18.1 (ATP-binding cassette, sub family D (ALD), member 3 (PMP70, PXMP1)) antibody
    • Peroxisomal membrane protein 1 (70kD) antibody
    • Peroxisomal membrane protein 1 (70kD, Zellweger syndrome) antibody
    • Peroxisomal membrane protein 1 antibody
    • PMP 70 antibody
    • PMP70 antibody
    • PXMP 1 antibody
    • PXMP1 antibody
    • ZWS2 antibody
    see all

Anti-PMP70 antibody images

  • Anti-PMP70 antibody (ab81904) at 0.2 µg/ml + Human Kidney Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-PMP70 antibody (ab81904) at 1/10000 dilution (in Licor Blocking Buffer for 2 hours at 25°C) + Nuclear lysate of Hela cells at 10 µg

    Secondary
    An Alexa Fluor®680-conjugated Donkey anti-goat IgG polyclonal at 1/10000 dilution

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab81904 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-PMP70 antibody (ab81904)

ab81904 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela cells)
Loading amount
10 µg
Specification
Hela cells
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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