Overview

  • Product nameAnti-PMP70 antibody (Biotin)
    See all PMP70 primary antibodies
  • Description
    Rabbit polyclonal to PMP70 (Biotin)
  • ConjugationBiotin
  • Specificityab28499 recognises Peroxisomal membrane protein 70 (PMP70).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Synthetic peptide:

    NYEFKKITEDTVEFGS

    , corresponding to C terminal amino acids 644 - 659 of Rat PMP70

  • Positive control
    • Mouse kidney protein extract

Properties

Applications

Our Abpromise guarantee covers the use of ab28499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 74 kDa.

Target

  • FunctionProbable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • Cellular localizationPeroxisome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa peroxisomal membrane protein antibody
    • ABC 43 antibody
    • ABC D3 antibody
    • ABC43 antibody
    • ABCD 3 antibody
    • Abcd3 antibody
    • ABCD3 protein antibody
    • ABCD3_HUMAN antibody
    • ATP binding cassette sub family D (ALD) member 3 antibody
    • ATP binding cassette sub family D member 3 antibody
    • ATP-binding cassette sub-family D member 3 antibody
    • Peroxisomal membrane protein 1 antibody
    • PMP 70 antibody
    • PMP70 antibody
    • PXMP 1 antibody
    • PXMP1 antibody
    • ZWS2 antibody
    see all

Anti-PMP70 antibody (Biotin) images

  • All lanes : Anti-PMP70 antibody (Biotin) (ab28499) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of PMP70 staining in A459 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in NS-1 cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in HMVEC cells with ab28499.

  • Immunofluorescent analysis of PMP70 staining in p19 cells with ab28499.

  • Flow cytometry analysis of PMP70 in HepG2 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 μg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow cytometry analysis of PMP70 in 293T cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

References for Anti-PMP70 antibody (Biotin) (ab28499)

This product has been referenced in:
  • Falcon A  et al. FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase. Am J Physiol Endocrinol Metab 299:E384-93 (2010). Purification ; Mouse . Read more (PubMed: 20530735) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"