Overview

  • Product name
    Anti-PNCK antibody
  • Description
    Rabbit polyclonal to PNCK
  • Specificity
    Detects endogenous levels of total PNCK protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human PNCK

  • Positive control
    • Human brain tissue LOVO cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab72514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by CAMKK1.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium/calmodulin dependent protein kinase type 1B antibody
    • Calcium/calmodulin-dependent protein kinase type 1B antibody
    • CaM KI beta antibody
    • CaM kinase I beta antibody
    • CaM kinase IB antibody
    • CaM-KI beta antibody
    • CaMK1b antibody
    • CaMKI beta antibody
    • CaMKI-beta antibody
    • EC 2.7.11.17 antibody
    • KCC1B_HUMAN antibody
    • MGC45419 antibody
    • PNCK antibody
    • Pregnancy up regulated non ubiquitously expressed CaM kinase antibody
    • Pregnancy up-regulated non-ubiquitously-expressed CaM kinase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab72514 at 1/50 dilution. Right panel was also treated with immunising peptide.
  • All lanes : Anti-PNCK antibody (ab72514) at 1/500 dilution

    Lane 1 : LOVO cell extract treated with hydrogen peroxide (100µM, 30 mins)
    Lane 2 : LOVO cell extract treated with hydrogen peroxide (100µM, 30 mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References

ab72514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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