Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50mM Tris buffered saline, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberPLY-4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 µg/ml.
Neutralising Use at an assay dependent concentration. PubMed: 8550195

Target

  • Cellular localizationPlasma membrane

Anti-Pneumolysin antibody [PLY-4] images

  • Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).

References for Anti-Pneumolysin antibody [PLY-4] (ab71810)

This product has been referenced in:
  • Li P  et al. Streptococcus pneumoniae induces autophagy through the inhibition of the PI3K-I/Akt/mTOR pathway and ROS hypergeneration in A549 cells. PLoS One 10:e0122753 (2015). Read more (PubMed: 25803050) »
  • Cassone M  et al. The HtrA Protease from Streptococcus pneumoniae Digests Both Denatured Proteins and the Competence-stimulating Peptide. J Biol Chem 287:38449-59 (2012). WB . Read more (PubMed: 23012372) »

See all 6 Publications for this product

Product Wall

Gracias por habernos contactado.
Le confirmo la información que me proporcionan mis compañeros del laboratorio: para inmunizar los ratones con los que se produjo ab71810 se utilizó PLY entera.
No dudes en contactarnos para cualquier otra cons...

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Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending two free of charge vials of ab71810 on the order ***, which should arrive tomorrow. I've requested that these vials be in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"