The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration. PubMed: 8550195
Cellular localizationPlasma membrane
Anti-Pneumolysin antibody [PLY-4] images
Western blot - Anti-Pneumolysin antibody [PLY-4] (ab71810)
Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).
References for Anti-Pneumolysin antibody [PLY-4] (ab71810)
This product has been referenced in:
Li P et al. Streptococcus pneumoniae induces autophagy through the inhibition of the PI3K-I/Akt/mTOR pathway and ROS hypergeneration in A549 cells. PLoS One10:e0122753 (2015).
Read more (PubMed: 25803050) »
Cassone M et al. The HtrA Protease from Streptococcus pneumoniae Digests Both Denatured Proteins and the Competence-stimulating Peptide. J Biol Chem287:38449-59 (2012).
Read more (PubMed: 23012372) »