Overview

  • Product name
    Anti-Pneumolysin antibody [PLY-4]
    See all Pneumolysin primary antibodies
  • Description
    Mouse monoclonal [PLY-4] to Pneumolysin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Neutralisingmore details
  • Immunogen

    Recombinant full length protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 50mM Tris buffered saline, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    PLY-4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 29636428
Neutralising Use at an assay dependent concentration. PubMed: 8550195

Target

  • Cellular localization
    Plasma membrane

Images

  • Coomassie Blue-stained gel (lane 1) and Western blots of purified recombinant PLY, 3 µg per lane, with mouse monoclonal antibodies PLY-4 (lane 2), PLY-5 (lane 3) and PLY-7 (lane 4); a PLY-irrelevant mouse monoclonal antibody 1.4G8.66 served as control (lane 5).

References

This product has been referenced in:
  • Codemo M  et al. Immunomodulatory Effects of Pneumococcal Extracellular Vesicles on Cellular and Humoral Host Defenses. MBio 9:N/A (2018). WB, ICC/IF . Read more (PubMed: 29636428) »
  • Shenoy AT  et al. Streptococcus pneumoniae in the heart subvert the host response through biofilm-mediated resident macrophage killing. PLoS Pathog 13:e1006582 (2017). Read more (PubMed: 28841717) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Streptococcus pneumoniae Cell lysate - whole cell (Streptococcus pneumoniae: serotype 3, strain A66)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Streptococcus pneumoniae: serotype 3, strain A66
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2017

Gracias por habernos contactado.
Le confirmo la información que me proporcionan mis compañeros del laboratorio: para inmunizar los ratones con los que se produjo ab71810 se utilizó PLY entera.
No dudes en contactarnos para cualquier otra cons...

Read More

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending two free of charge vials of ab71810 on the order ***, which should arrive tomorrow. I've requested that these vials be in...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up