Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab168473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • Function
    Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
  • Tissue specificity
    Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
  • Involvement in disease
    Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
  • Sequence similarities
    In the N-terminal section; belongs to the DNA 3' phosphatase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2''(3'')-polynucleotidase antibody
    • 2'(3')-polynucleotidase antibody
    • Bifunctional polynucleotide phosphatase/kinase antibody
    • DEM 1 antibody
    • DEM1 antibody
    • DNA 5' kinase/3' phosphatase antibody
    • DNA 5''-kinase/3''-phosphatase antibody
    • EIEE10 antibody
    • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
    • MCSZ antibody
    • PNK 1 antibody
    • PNK1 antibody
    • Pnkp antibody
    • PNKP DNA kinase antibody
    • PNKP_HUMAN antibody
    • Polynucleotide 3'-phosphatase antibody
    • Polynucleotide 5' hydroxyl kinase antibody
    • Polynucleotide 5''-hydroxyl-kinase antibody
    • Polynucleotide kinase 3 prime phosphatase antibody
    • Polynucleotide kinase 3' phosphatase antibody
    • Polynucleotide Kinase antibody
    • Polynucleotide kinase-3''-phosphatase antibody
    see all

Images

  • All lanes : Anti-PNK antibody (ab168473) at 1 µg/ml

    Lane 1 : PNK transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.


    Predicted band size : 57 kDa
  • Anti-PNK antibody (ab168473) at 1 µg/ml + Human spleen lysate at 50 µg

    Predicted band size : 57 kDa

References

ab168473 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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