Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB on fusion protein: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).


    This antibody has been used to successfully detect PNK as a fusion protein in a yeast lysate (see picture below). However, it has not been successfully used to detect endogenous PNK in cell lines (HepG2, HeLa and 293).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    • Function
      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
    • Tissue specificity
      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
    • Involvement in disease
      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
    • Sequence similarities
      In the N-terminal section; belongs to the DNA 3' phosphatase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2''(3'')-polynucleotidase antibody
      • 2'(3')-polynucleotidase antibody
      • Bifunctional polynucleotide phosphatase/kinase antibody
      • DEM 1 antibody
      • DEM1 antibody
      • DNA 5' kinase/3' phosphatase antibody
      • DNA 5''-kinase/3''-phosphatase antibody
      • EIEE10 antibody
      • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
      • MCSZ antibody
      • PNK 1 antibody
      • PNK1 antibody
      • Pnkp antibody
      • PNKP DNA kinase antibody
      • PNKP_HUMAN antibody
      • Polynucleotide 3'-phosphatase antibody
      • Polynucleotide 5' hydroxyl kinase antibody
      • Polynucleotide 5''-hydroxyl-kinase antibody
      • Polynucleotide kinase 3 prime phosphatase antibody
      • Polynucleotide kinase 3' phosphatase antibody
      • Polynucleotide Kinase antibody
      • Polynucleotide kinase-3''-phosphatase antibody
      see all

    Images



    • Predicted band size : 58 kDa

      This image is courtesy of Keith Caldecott, University of Sussex

      Western blot using ab3817 at 1/1000 incubated for 1hr in TBST.

      Lane 1: Yeast Extract

      Lane 2:  Yeast Extract + AD-PNK human fusion protein

      The AD fusion is an Activation Domain fusion protein.

      The band representing PNK is marked by the arrow.

      Western blot using ab3817 at 1/1000 incubated for 1hr in TBST.

      Lane 1: Yeast Extract
      Lane 2: Yeast Extract + AD-PNK human fusion protein

      The AD fusion is an Activation Domain fusion protein.

      The band representing PNK is marked by the arrow.

    References

    This product has been referenced in:
    • Della-Maria J  et al. The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites. J Biol Chem 287:39233-44 (2012). WB . Read more (PubMed: 22992732) »
    • Smith AP  et al. A positive role for Myc in TGFbeta-induced Snail transcription and epithelial-to-mesenchymal transition. Oncogene 28:422-30 (2009). Read more (PubMed: 18978814) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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