The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB on fusion protein: 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
This antibody has been used to successfully detect PNK as a fusion protein in a yeast lysate (see picture below). However, it has not been successfully used to detect endogenous PNK in cell lines (HepG2, HeLa and 293).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
Tissue specificityExpressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
Involvement in diseaseDefects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
Sequence similaritiesIn the N-terminal section; belongs to the DNA 3' phosphatase family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
PNK 1 antibody
PNKP DNA kinase antibody
Polynucleotide 3'-phosphatase antibody
Polynucleotide 5' hydroxyl kinase antibody
Polynucleotide 5''-hydroxyl-kinase antibody
Polynucleotide kinase 3 prime phosphatase antibody
Polynucleotide kinase 3' phosphatase antibody
Polynucleotide Kinase antibody
Polynucleotide kinase-3''-phosphatase antibody
Anti-PNK antibody images
Western blot - PNK antibody (ab3817)
Predicted band size : 58 kDa
Western blot using ab3817 at 1/1000 incubated for 1hr in TBST.
Lane 1: Yeast Extract
Lane 2: Yeast Extract + AD-PNK human fusion protein
The AD fusion is an Activation Domain fusion protein.
The band representing PNK is marked by the arrow.
Keith Caldecott, University of Sussex
References for Anti-PNK antibody (ab3817)
This product has been referenced in:
Della-Maria J et al. The Interaction between Polynucleotide Kinase Phosphatase and the DNA Repair Protein XRCC1 Is Critical for Repair of DNA Alkylation Damage and Stable Association at DNA Damage Sites. J Biol Chem287:39233-44 (2012).
Read more (PubMed: 22992732) »