Overview

  • Product name
  • Description
    Rabbit polyclonal to PNK
  • Specificity
    This antibody detects a band corresponding to PNK in an EM9 cell (hamster) lysate overexpressing PNK, it does not detect this band in a wildtype EM9 cell lysate. It detects a band of the same size in Hela cell lysate.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RTPESQPDTPPGC

    , corresponding to amino acids 110 - 121 of Human PNK.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
  • Tissue specificity
    Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
  • Involvement in disease
    Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
  • Sequence similarities
    In the N-terminal section; belongs to the DNA 3' phosphatase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2''(3'')-polynucleotidase antibody
    • 2'(3')-polynucleotidase antibody
    • Bifunctional polynucleotide phosphatase/kinase antibody
    • DEM 1 antibody
    • DEM1 antibody
    • DNA 5' kinase/3' phosphatase antibody
    • DNA 5''-kinase/3''-phosphatase antibody
    • EIEE10 antibody
    • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
    • MCSZ antibody
    • PNK 1 antibody
    • PNK1 antibody
    • Pnkp antibody
    • PNKP DNA kinase antibody
    • PNKP_HUMAN antibody
    • Polynucleotide 3'-phosphatase antibody
    • Polynucleotide 5' hydroxyl kinase antibody
    • Polynucleotide 5''-hydroxyl-kinase antibody
    • Polynucleotide kinase 3 prime phosphatase antibody
    • Polynucleotide kinase 3' phosphatase antibody
    • Polynucleotide Kinase antibody
    • Polynucleotide kinase-3''-phosphatase antibody
    see all

Images

  • Lane 1:  Y190 yeast cell lysate, 10 µg.
    Lane 2:  Y190 yeast cell lysate + human PNK (Gal DNA BP), 10 µg.
    Lane 3:  EM9 XH chinese hamster ovary cell lysate, 10 µg.   
    Lane 4:  EM9 XH chinese hamster ovary cell lysate + human PNK, 10 µg.
    Lane 5:  HeLa cell lysate, 10 µg. 

    ab4191 was incubated for 1-2 hours in TBST at a dilution of 1/1000.

References

ab4191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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