Recombinant
RabMAb

Anti-PNK antibody [EPR11934(B)] (ab170954)

Overview

  • Product name
    Anti-PNK antibody [EPR11934(B)]
    See all PNK primary antibodies
  • Description
    Rabbit monoclonal [EPR11934(B)] to PNK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PNK aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control
    • HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11934(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
    • Tissue specificity
      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
    • Involvement in disease
      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
    • Sequence similarities
      In the N-terminal section; belongs to the DNA 3' phosphatase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2''(3'')-polynucleotidase antibody
      • 2'(3')-polynucleotidase antibody
      • Bifunctional polynucleotide phosphatase/kinase antibody
      • DEM 1 antibody
      • DEM1 antibody
      • DNA 5' kinase/3' phosphatase antibody
      • DNA 5''-kinase/3''-phosphatase antibody
      • EIEE10 antibody
      • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
      • MCSZ antibody
      • PNK 1 antibody
      • PNK1 antibody
      • Pnkp antibody
      • PNKP DNA kinase antibody
      • PNKP_HUMAN antibody
      • Polynucleotide 3'-phosphatase antibody
      • Polynucleotide 5' hydroxyl kinase antibody
      • Polynucleotide 5''-hydroxyl-kinase antibody
      • Polynucleotide kinase 3 prime phosphatase antibody
      • Polynucleotide kinase 3' phosphatase antibody
      • Polynucleotide Kinase antibody
      • Polynucleotide kinase-3''-phosphatase antibody
      see all

    Images

    • ab170954 showing +ve staining in Human testis tissue.

    • ab170954 showing +ve staining in Human normal pancreas tissue.

    • ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK using ab170954 at a 1/100 dilution.

      The use of an HRP/AP polymerized secondary antibody is recommended.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK using ab170954 at a 1/100 dilution.

      The use of an HRP/AP polymerized secondary antibody is recommended.

    • Immunofluorescence analysis of PC3 cells, labeling PNK using ab170954 at a 1/100 dilution.

    • All lanes : Anti-PNK antibody [EPR11934(B)] (ab170954) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 57 kDa

    References

    ab170954 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170954.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up