Overview

  • Product nameAnti-PNPLA3 antibody
    See all PNPLA3 primary antibodies
  • Description
    Rabbit polyclonal to PNPLA3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PNPLA3 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab102097)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Adipose Tissue; Human Liver Tissue; HepG2 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

Target

  • RelevancePNPLA3 is a multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. It contains one patatin domain.
  • Cellular localizationMembrane; Single pass type II membrane protein
  • Database links
  • Alternative names
    • Acylglycerol O acyltransferase antibody
    • Adiponutrin antibody
    • ADPN antibody
    • C22orf20 antibody
    • Calcium independent phospholipase A2 epsilon antibody
    • DJ796I17.1 antibody
    • iPLA(2)epsilon antibody
    • IPLA2-epsilon antibody
    • Patatin like phospholipase domain containing 3 antibody
    • patatin like phospholipase domain containing protein 3 antibody
    • phospholipase A2, calcium independent, epsilon antibody
    see all

Anti-PNPLA3 antibody images

  • All lanes : Anti-PNPLA3 antibody (ab81874) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,140 kDa,30 kDa,44 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab81874 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81874, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and HepG2 cells at 5µg/ml.

References for Anti-PNPLA3 antibody (ab81874)

This product has been referenced in:
  • Xu F  et al. SIRT1 mediates the effect of GLP-1 receptor agonist exenatide on ameliorating hepatic steatosis. Diabetes 63:3637-46 (2014). WB ; Mouse . Read more (PubMed: 24947350) »
  • Hao L  et al. Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells. Metabolism 63:1352-62 (2014). WB ; Mouse . Read more (PubMed: 25060692) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Cow Tissue lysate - other (Adipose tissue)
Gel Running Conditions Non-reduced Denaturing (4-12%)
Loading amount 25 µg
Specification Adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Sophia Erb

Verified customer

Submitted Aug 18 2016

Thank you for your interest in our antibodies. We unfortunately do not have any small samplers of any of our antibodies. I suggest trying ab81874. We have not tested it for reactivity with mouse samples, but the immunogen has 93% identity with mouse PN...

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DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab81874 inWB on bovine. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abrev...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"