Overview

  • Product nameAnti-PNPLA8 antibody
    See all PNPLA8 primary antibodies
  • Description
    Rabbit polyclonal to PNPLA8
  • SpecificityThis antibody detects endogenous levels of total PNPLA8 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from the internal region of human PNPLA8.

  • Positive control
    • Extracts from Hela cells, A549 cells and HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/40000.
    WB: 1/500 - 1/1000. Detects bands of approximately 34, 48 and 88 kDa (predicted molecular weight: 88 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids.
    • Tissue specificityExpressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart.
    • Sequence similaritiesContains 1 patatin domain.
    • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasm > perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium-independent phospholipase A2-gamma antibody
      • Intracellular membrane associated calcium independent phospholipase A2 gamma antibody
      • Intracellular membrane-associated calcium-independent phospholipase A2 gamma antibody
      • IPLA2 2 antibody
      • iPLA2 gamma antibody
      • IPLA2(GAMMA) antibody
      • iPLA2-2 antibody
      • iPLA2-gamma antibody
      • IPLA22 antibody
      • IPLA2G antibody
      • Patatin like phospholipase domain containing protein 8 antibody
      • Patatin-like phospholipase domain-containing protein 8 antibody
      • PLPL8_HUMAN antibody
      • PNPLA-gamma antibody
      • PNPLA8 antibody
      see all

    Anti-PNPLA8 antibody images

    • All lanes : Anti-PNPLA8 antibody (ab74242) at 1/500 dilution

      Lane 1 : Extracts from Hela cells
      Lane 2 : Extracts from A549 cells
      Lane 3 : Extracts from HUVEC cells
      Lane 4 : Extracts from HUVEC cells plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 88 kDa
      Observed band size : 88 kDa
      Additional bands at : 34 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PNPLA8 antibody (ab74242)

    ab74242 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab74242.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"