Overview

  • Product nameAnti-PNPO antibody [1H9]
    See all PNPO primary antibodies
  • Description
    Mouse monoclonal [1H9] to PNPO
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of human PNPO produced in HEK293T cells (NP_060599).

  • Positive control
    • HEK293T cells transfected with PNPO, Human breast, Human Kidney, Carcinoma of Human liver, and Human thyroid tissues
  • General notesDilute in PBS (pH7.3) before use.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 30 kDa.
IHC-P 1/150.

Target

  • FunctionCatalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
  • PathwayCofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.
    Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
  • Involvement in diseaseDefects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency) [MIM:610090]; also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine.
  • Sequence similaritiesBelongs to the pyridoxamine 5'-phosphate oxidase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 1.4.3.5 antibody
    • FLJ10535 antibody
    • PDXPO antibody
    • PNPO antibody
    • PNPO_HUMAN antibody
    • Pyridoxal 5' phosphate synthase antibody
    • pyridoxamine 5' phosphate oxidase antibody
    • Pyridoxamine phosphate oxidase antibody
    • Pyridoxamine-phosphate oxidase antibody
    • pyridoxine 5' phosphate oxidase antibody
    • Pyridoxine-5'-phosphate oxidase antibody
    see all

Anti-PNPO antibody [1H9] images

  • All lanes : Anti-PNPO antibody [1H9] (ab119395) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with the pCMV6-ENTRY PNPO

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 30 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200133)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue using ab119395 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119395 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using ab119395 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human thyroid tissue using ab119395 at a dilution of 1/150.

References for Anti-PNPO antibody [1H9] (ab119395)

ab119395 has not yet been referenced specifically in any publications.

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