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Product name Anti-PNPO antibody See all PNPO primary antibodies
Mouse polyclonal to PNPO
Tested applications Suitable for:
WB more details
PNPO (NP_060599.1, 1 a.a. ~ 261 a.a) full-length human protein.
transfected 293T cell lysate
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: Whole serum
Purity Whole antiserum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 30 kDa).
Function Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
Pathway Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Involvement in disease Defects in PNPO are the cause of pyridoxine-5'-phosphate oxidase deficiency (PNPO deficiency) [MIM:610090]; also known as PNPO-related neonatal epileptic encephalopathy. The main feature of neonatal epileptic encephalopathy is the onset within hours of birth of a severe seizure disorder that does not respond to anticonvulsant drugs and can be fatal. Seizures can cease with the administration of PLP, being resistant to treatment with pyridoxine.
Sequence similarities Belongs to the pyridoxamine 5'-phosphate oxidase family.
Information by UniProt
EC 18.104.22.168 antibody
Anti-PNPO antibody images
Western blot - PNPO antibody (ab72647)
All lanes : Anti-PNPO antibody (ab72647) at 1/500 dilution Lane 1 : PNPO-transfected 293T cell line lysate Lane 2 : Non-transfected 293T cell line lysate Secondary HRP-conjugated goat anti-mouse IgG at 1/2500 dilution Predicted band size : 30 kDa Observed band size : 27 kDa ( why is the actual band size different from the predicted?)
References for Anti-PNPO antibody (ab72647)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"