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Full length protein corresponding to Podoplanin/ gp36. MDCK cells stably expressing Podoplanin/ gp36 protein.
Database link: Q86YL7
Our Abpromise guarantee covers the use of ab10288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 6 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).|
|Flow Cyt||Use 1µl for 106 cells.
(methanol fixed cells)
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18987360|
ab10288 staining Podoplanin / gp36 in human differentiated cardiac ventricular cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/300) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat monoclonal anti-mouse IgG (1/1500) was used as the secondary antibody.
Gating Strategy: Isotype negative control (white).
ab10288 at a 1/500 dilution staining human dermal lymphatic endothelial by Immunocytochemistry. The cells were grown in coverslips and fixed in formalin prior to permeabilization with tritonX-100. The cells were incubated with the primary antibody at 1/500 for 1 hour and then bound antibody was detected using a biotinylated goat anti-mouse antibody. Texas red-streptavidin was used for development.
This image is courtesy of an Abreview submitted by Maria Navarro.
Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) - lane 1, and HUVECs - lane 2. Total lysate of both cell types were subjected to SDS-PAGE and subsequent western blot analysis with ab10288. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
Immunocytochemistry/Immunofluorescence analysis of human dermal lymphatic endothelial cells (HDLEC) labelling Podoplanin / gp36 with ab10288. An Alexa Fluor® 488-conjugated goat anti-mouse was used as the secondary antibody. Nuclei counterstained with DAPI.
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