Anti-Podoplanin / gp36 antibody [8.1.1] (ab92319)

Overview

  • Product nameAnti-Podoplanin / gp36 antibody [8.1.1]
    See all Podoplanin / gp36 primary antibodies
  • Description
    Syrian Hamster monoclonal [8.1.1] to Podoplanin / gp36
  • Tested applicationsSuitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (mouse): Thymic epithelial cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab92319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IP
  • Application notesIHC-Fr: Use at a concentration of 0.5 - 1 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
    • Tissue specificityHighly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
    • Sequence similaritiesBelongs to the podoplanin family.
    • Post-translational
      modifications
      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.
    • Cellular localizationMembrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aggrus antibody
      • Glycoprotein 36 KD antibody
      • Glycoprotein 36 antibody
      • gp 36 antibody
      • GP 38 antibody
      • GP 40 antibody
      • gp36 antibody
      • GP38 antibody
      • GP40 antibody
      • HT1A 1 antibody
      • HT1A1 antibody
      • hT1alpha 1 antibody
      • hT1alpha 2 antibody
      • hT1alpha1 antibody
      • hT1alpha2 antibody
      • Lung type I cell membrane associated glycoprotein antibody
      • Lung type I cell membrane associated glycoprotein isoform a antibody
      • Lung type I cell membrane associated glycoprotein T1A 2 antibody
      • OTS 8 antibody
      • OTS8 antibody
      • OTTHUMP00000009640 antibody
      • OTTHUMP00000044504 antibody
      • PA2.26 antibody
      • PA2.26 antigen antibody
      • Pdpn antibody
      • PDPN_HUMAN antibody
      • Podoplanin antibody
      • PSEC0003 antibody
      • PSEC0025 antibody
      • T1 alpha antibody
      • T1 ALPHA GENE antibody
      • T1-alpha antibody
      • T1A 2 antibody
      • T1A antibody
      • TI1A antibody
      • TIA 2 antibody
      • TIA2 antibody
      see all

    Anti-Podoplanin / gp36 antibody [8.1.1] images

    • Frozen section (7.5 micron) of murine kidney were incubated with ab92319 antibody at 1ug/ml and detected with an Alexa Fluor®488-conjugated Goat anti-hamster IgG polyclonal used at 1:1000 dilution. Exposure time 500 ms.

    References for Anti-Podoplanin / gp36 antibody [8.1.1] (ab92319)

    This product has been referenced in:
    • Farr A  et al. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem 40:651-64 (1992). Read more (PubMed: 1374092) »
    • Farr AG  et al. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med 176:1477-82 (1992). Read more (PubMed: 1402691) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry and your interest in our products.

    I can confirm that the clone number of ab11936 is RTD4E10 which can be found in the brackets of the target name as well as on the on-line product datasheet under the clone number. ...

    Read More

    The host is Syrian/Golden Hamster. I have updated the datasheet. My apologies for the inconvenience. There are many options for anti-Syrian Hamster IgG. Please see the Secondary antibodies- Advance search: http://www.abcam.com/index.html?pageconf...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"