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- Specific References
- Protocols
Overview
- Product nameAnti-PODXL antibody
See all PODXL primary antibodies - DescriptionRabbit polyclonal to PODXL
- Tested applicationsSuitable for: WB, ELISAmore details
- Species reactivityReacts with: Human
- Immunogen
A region within synthetic peptide: RGGGSGNPTT TIESPKSTKS ADTTTVATST ATAKPNTTSS QNGAEDTTNS, corresponding to N terminal amino acids 109-158 of Human PODXL
- Positive control
- HepG2 cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab62594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1:312500. |
Target
- FunctionInvolved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
- Tissue specificityGlomerular epithelium cell (podocyte).
- Sequence similaritiesBelongs to the podocalyxin family.
- DomainBoth the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with SLC9A3R1 and for targeting SLC9A3R1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity). The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes.
- Post-translational
modificationsN- and O-linked glycosylated. Sialoglycoprotein. - Cellular localizationApical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane. In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).
- Information by UniProt
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Database links
- Entrez Gene: 5420 Human
- Omim: 602632 Human
- SwissProt: O00592 Human
- Unigene: 744213 Human
- FormThere are 2 isoforms produced by alternative splicing.
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Alternative names
- GCTM-2 antigen antibody
- Gp2 antibody
- Gp200 antibody
see all
Anti-PODXL antibody images
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Western blot - PODXL antibody (ab62594)Anti-PODXL antibody (ab62594) at 5 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
References for Anti-PODXL antibody (ab62594)
ab62594 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"