Overview

  • Product name
  • Description
    Rabbit polyclonal to Pofut1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Pofut1 (Uniprot ID: Q9H488).

  • Positive control
    • HCT116, Mouse liver and HUVEC whole cell lysates; HCT116 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • Tissue specificity
    Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 68 family.
  • Cellular localization
    Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

Images

  • Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    12% SDS PAGE
  • Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Anti-Pofut1 antibody (ab154051) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg

    Predicted band size: 44 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HCT116 cells, labeling Pofut1 with ab154051 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab154051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154051.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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