Overview

  • Product name
    Anti-POGK antibody
  • Description
    Rabbit polyclonal to POGK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human POGK (NP_060012.3).

  • Positive control
    • HeLa, 293T or Jurkat whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities
    Contains 1 DDE domain.
    Contains 1 HTH CENPB-type DNA-binding domain.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BASS 2 antibody
    • BASS2 antibody
    • KIAA1513 antibody
    • KIAA15131 antibody
    • LST003 antibody
    • POGK antibody
    • POGK_HUMAN antibody
    • Pogo transposable element with KRAB domain antibody
    • SLTP003 antibody
    see all

Images

  • All lanes : Anti-POGK antibody (ab115190) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg


    Predicted band size : 69 kDa
  • Samples: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
    Antibodies: ab115190 used for WB at 0.1 µg/ml and used for IP at 6 µg/mg lysate.
    Detection: chemiluminescence with exposure time of 30 seconds (B).

References

ab115190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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