Recombinant
RabMAb

Anti-POGZ antibody [EPR10612] (ab167408)

Overview

  • Product name
    Anti-POGZ antibody [EPR10612]
    See all POGZ primary antibodies
  • Description
    Rabbit monoclonal [EPR10612] to POGZ
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human POGZ aa 1-100.

  • Positive control
    • HepG2 and SH-SY5Y cell lysates; Human cervical carcinoma and Human prostate tissues; HepG2 and SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10612
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab167408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 155 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
    • Sequence similarities
      Contains 9 C2H2-type zinc fingers.
      Contains 1 DDE domain.
      Contains 1 HTH CENPB-type DNA-binding domain.
    • Cellular localization
      Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0461 antibody
      • MGC71543 antibody
      • Pogo transposable element with ZNF domain antibody
      • POGZ antibody
      • POGZ_HUMAN antibody
      • putative protein product of Nbla00003 antibody
      • SUHW5 antibody
      • suppressor of hairy wing homolog antibody
      • Suppressor of hairy wing homolog 5 antibody
      • Zinc finger protein 280E antibody
      • Zinc finger protein 635 antibody
      • ZNF280E antibody
      • ZNF635 antibody
      • ZNF635M antibody
      see all

    Images

    • ab167408 showing +ve staining in Human normal colon.
    • ab167408 showing +ve staining in Human breast carcinoma.
    • ab167408 showing +ve staining in Human ovarian carcinoma.
    • All lanes : Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 155 kDa
    • Flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).
    • Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.

    References

    ab167408 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab167408.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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