Overview

  • Product nameAnti-POLDIP1 antibody
    See all POLDIP1 primary antibodies
  • Description
    Rabbit polyclonal to POLDIP1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from a region between amino acids 26-75 of Human POLDIP1. Note: The exact sequence is proprietary.

  • Positive control
    • WB: Fetal heart lysate. IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab32974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • Tissue specificityExpressed in a wide variety of tissues.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BACURD family.
    Contains 1 BTB (POZ) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BACD1_HUMAN antibody
    • BACURD1 antibody
    • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 antibody
    • BTB/POZ domain-containing protein KCTD13 antibody
    • FKSG86 antibody
    • hBACURD1 antibody
    • KCTD13 antibody
    • PDIP1 antibody
    • POLDIP 1 antibody
    • POLDIP1 antibody
    • Polymerase delta-interacting protein 1 antibody
    • Potassium channel tetramerisation domain containing 13 antibody
    • PP6832 antibody
    • TNFAIP1 like polymerase delta interacting protein 1 antibody
    • TNFAIP1-like protein antibody
    see all

Anti-POLDIP1 antibody images

  • Anti-POLDIP1 antibody (ab32974) at 0.25 µg/ml + fetal heart lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling POLDIP1 with ab32974 at 4-8µg/ml. Cells with positive label (indicated by arrows) are neural cells. Magnification: 400X.

References for Anti-POLDIP1 antibody (ab32974)

This product has been referenced in:
  • Werwein E  et al. B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1). Cell Cycle 11:4047-58 (2012). Read more (PubMed: 23032261) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"