The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Tissue specificityExpressed in a wide variety of tissues.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BACURD family. Contains 1 BTB (POZ) domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling POLDIP1 with ab32974 at 4-8µg/ml. Cells with positive label (indicated by arrows) are neural cells. Magnification: 400X.
References for Anti-POLDIP1 antibody (ab32974)
This product has been referenced in:
Werwein E et al. B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1). Cell Cycle11:4047-58 (2012).
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