The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionParticipates in DNA repair and in chromosomal DNA replication.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
DomainThe DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.