Overview

  • Product name
  • Description
    Mouse polyclonal to POLE
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human, aa 1-371)

Properties

Applications

Our Abpromise guarantee covers the use of ab67420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Function
    Participates in DNA repair and in chromosomal DNA replication.
  • Sequence similarities
    Belongs to the DNA polymerase type-B family.
  • Domain
    The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRCS12 antibody
    • DKFZp434F222 antibody
    • DNA polymerase epsilon catalytic subunit A antibody
    • DNA polymerase epsilon catalytic subunit antibody
    • DNA polymerase II subunit A antibody
    • DPOE1_HUMAN antibody
    • EC 2.7.7.7 antibody
    • FILS antibody
    • FLJ21434 antibody
    • POLE antibody
    • POLE1 antibody
    • Polymerase (DNA directed) epsilon antibody
    • polymerase (DNA directed), epsilon, catalytic subunit antibody
    see all

Images

  • All lanes : Anti-POLE antibody (ab67420) at 1/500 dilution

    Lane 1 : POLE transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

References

ab67420 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab67420.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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