Anti-Poliovirus Receptor antibody (ab103630)

Overview

  • Product nameAnti-Poliovirus Receptor antibody
    See all Poliovirus Receptor primary antibodies
  • Description
    Rabbit polyclonal to Poliovirus Receptor
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-417 of Human Poliovirus Receptor (NP_006496.2).

  • Positive control
    • Mouse stomach tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ICC/IF 1/200.

Target

  • FunctionMediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • Sequence similaritiesBelongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD155 antibody
    • CD155 antigen antibody
    • FLJ25946 antibody
    • HVED antibody
    • mE4 antibody
    • NECL 5 antibody
    • Necl-5 antibody
    • Necl5 antibody
    • Nectin like 5 antibody
    • Nectin like protein 5 antibody
    • Nectin-like protein 5 antibody
    • Ortholog of mouse Tage4 antibody
    • Poliovirus receptor antibody
    • PVR antibody
    • PVR_HUMAN antibody
    • PVS antibody
    • Taa1 antibody
    • Tage 4 antibody
    • TAGE4 antibody
    see all

Anti-Poliovirus Receptor antibody images

  • Anti-Poliovirus Receptor antibody (ab103630) at 1/500 dilution + Mouse stomach tissue lysate at 50 µg

    Predicted band size : 45 kDa
  • All lanes : Anti-Poliovirus Receptor antibody (ab103630) at 1/500 dilution

    Lane 1 : Poliovirus Receptor transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 45 kDa
  • Immunofluorescence analysis of murine stomach, staining Poliovirus Receptor with ab103630.

    Cells were fixed with acetone and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 24 hours at 4°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/3000) was used as the secondary antibody.

    See Abreview

References for Anti-Poliovirus Receptor antibody (ab103630)

ab103630 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing
Sample Pig Cell lysate - whole cell (Swine kidney)
Specification Swine kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Korneel Grauwet

Verified customer

Submitted Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Stomach)
Specification Stomach
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

DISCOUNT CODE: 42351

Expiration date: 27/12/2012


I am very pleased to hear you would like to accept our offer and testab103630 in IHC-Fr on Ms stomack. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF O...

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Thank you for your answer.

It is very important to design a good control experiments when two untested factors are involved. Could you please confirm ifyou are going to useformalin-fixed paraffin-embedded(IHC-P) or frozen tissue (IHC-Fr) sect...

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Thank you for getting back to me.

This antibody has only been tested in Western blot application on tissue lysate of mouse stomach and transfected human cells (293T). It is very important to control the testing experiments to make sure that t...

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Thank you for your enquiry and your interest in our products.

This antibody (ab103630: Anti-Poliovirus Receptor antibody) has not been tested in rat neither in immunohistochemistry.

We can offer testing discount only for either new ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"