Purification notesab60061 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 15 kDa).
Use at an assay dependent dilution.
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.
Involvement in diseaseTreacher Collins syndrome 2
Sequence similaritiesBelongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.