Anti-POLR1D antibody (ab104115)
Key features and details
- Rabbit polyclonal to POLR1D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR1D antibody
See all POLR1D primary antibodies -
Description
Rabbit polyclonal to POLR1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-133 of Human POLR1D (AAH00889).
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Positive control
- A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, HCT116 cell lysate, HBC435 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 15 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 15 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Disease: Treacher Collins syndrome 2 Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. Similarity: Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. -
Database links
- Entrez Gene: 539061 Cow
- Entrez Gene: 51082 Human
- Entrez Gene: 20018 Mouse
- Omim: 613715 Human
- SwissProt: Q1RMG8 Cow
- SwissProt: P0DPB5 Human
- SwissProt: P0DPB6 Human
- SwissProt: Q9Y2S0 Human
see all -
Alternative names
- AC19 antibody
- DNA-directed RNA polymerase I subunit D antibody
- DNA-directed RNA polymerases I and III subunit RPAC2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab104115 has been referenced in 1 publication.
- Salva JE et al. Nuclear FGFR2 regulates musculoskeletal integration within the developing limb. Dev Dyn 248:233-246 (2019). PubMed: 30620790