Overview

  • Product nameAnti-POLR1D antibody
    See all POLR1D primary antibodies
  • Description
    Rabbit polyclonal to POLR1D
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: IQTRGTLPAV EPFQRGLNEL MNVCQHVLDK FEASIKDYKD QKASRNESTF , corresponding to internal sequence amino acids 84-133 of Human POLR1D

  • Positive control
    • Fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.
  • Involvement in diseaseTreacher Collins syndrome 2
  • Sequence similaritiesBelongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC19 antibody
    • DNA directed RNA polymerase I 16 kDa polypeptide antibody
    • DNA directed RNA polymerase I subunit D antibody
    • DNA directed RNA polymerases I and III subunit RPAC2 antibody
    • DNA-directed RNA polymerase I subunit D antibody
    • DNA-directed RNA polymerases I and III subunit RPAC2 antibody
    • FLJ20616 antibody
    • hRPA19 antibody
    • MGC9850 antibody
    • POLR1C antibody
    • POLR1D antibody
    • Polymerase (RNA) I polypeptide D antibody
    • Polymerase (RNA) I polypeptide D, 16kDa antibody
    • RNA polymerase I 16 kDa subunit antibody
    • RNA polymerases I and III subunit AC2 antibody
    • RPA16 antibody
    • RPA9 antibody
    • RPAC2 antibody
    • RPAC2_HUMAN antibody
    • RPC16 antibody
    • RPO1 3 antibody
    see all

Anti-POLR1D antibody images

  • Anti-POLR1D antibody (ab80936) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-POLR1D antibody (ab80936)

ab80936 has not yet been referenced specifically in any publications.

Product Wall

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