Overview

  • Product nameAnti-POLR2G antibody [2781C2a]
    See all POLR2G primary antibodies
  • Description
    Mouse monoclonal [2781C2a] to POLR2G
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment from human POLR2G, corresponding to an internal sequence.

  • Positive control
    • HT-1080, NIH3T3 and F2408 whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notesWB: 1/50. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
    Dot: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.
    • Sequence similaritiesBelongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA directed RNA polymerase II 19 kDa polypeptide antibody
      • DNA directed RNA polymerase II subunit G antibody
      • DNA directed RNA polymerase II subunit RPB7 antibody
      • DNA-directed RNA polymerase II subunit G antibody
      • DNA-directed RNA polymerase II subunit RPB7 antibody
      • hRPB19 antibody
      • hsRPB7 antibody
      • MGC138367 antibody
      • MGC138369 antibody
      • POLR2G antibody
      • polymerase (RNA) II (DNA directed) polypeptide G antibody
      • RNA polymerase II 19 kDa subunit antibody
      • RNA polymerase II subunit B7 antibody
      • RPB19 antibody
      • RPB7 antibody
      • RPB7_HUMAN antibody
      see all

    Anti-POLR2G antibody [2781C2a] images

    • Anti-POLR2G antibody [2781C2a] (ab53742) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 19 kDa
    • Anti-POLR2G antibody [2781C2a] (ab53742) at 1/50 dilution + F2408 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 19 kDa
    • Anti-POLR2G antibody [2781C2a] (ab53742) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 19 kDa
      Observed band size : 19 kDa

    References for Anti-POLR2G antibody [2781C2a] (ab53742)

    ab53742 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab53742.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"