Overview

  • Product nameAnti-POLR2J2 antibody
  • Description
    Mouse monoclonal to POLR2J2
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-115 of Human POLR2J2 with a proprietary tag (NP_116581).

  • Positive control
    • Recombinant Human POLR2J2 Protein; POLR2J2 transfected 293T cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab118114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the archaeal rpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II polypeptide J-related antibody
    • DNA-directed RNA polymerase II subunit J2 antibody
    • DNA-directed RNA polymerase II subunit RPB11-b1 antibody
    • HRPB11B antibody
    • MGC105050 antibody
    • MGC54043 antibody
    • OTTHUMP00000216459 antibody
    • POLR2J2 antibody
    • Polymerase (RNA) II (DNA directed) polypeptide J2 antibody
    • RNA polymerase II subunit B11-b1 antibody
    • RPB11b1 antibody
    • RPB1B_HUMAN antibody
    see all

Anti-POLR2J2 antibody images

  • Anti-POLR2J2 antibody (ab118114) at 5 µg/ml + recombinant immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 13 kDa
  • All lanes : Anti-POLR2J2 antibody (ab118114) at 5 µg/ml

    Lane 1 : POLR2J2 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 13 kDa
  • ab118114 at 10 µg/ml as a capture antibody. The detection limit for recombinant tagged POLR2J2 is 0.3 ng/ml.

References for Anti-POLR2J2 antibody (ab118114)

ab118114 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"