DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Expressed in the brain, in the cortex and the white matter (at protein level).
Involvement in disease
Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
DNA directed RNA polymerase III largest subunit antibody
DNA directed RNA polymerase III subunit A antibody
DNA-directed RNA polymerase III largest subunit antibody
DNA-directed RNA polymerase III subunit A antibody
DNA-directed RNA polymerase III subunit RPC1 antibody
POLR 3A antibody
Polymerase (RNA) III (DNA directed) polypeptide A 155kDa antibody
Polymerase (RNA) III (DNA directed) polypeptide A antibody
RNA polymerase III 155 kDa subunit antibody
RNA polymerase III subunit C1 antibody
RNA polymerase III subunit C160 antibody
RNA polymerase III subunit RPC155 D antibody
ChIP - Anti-POLR3A antibody - ChIP Grade (ab96328)This image is courtesy of an Abreview submitted by Jennifer Blancas
ChIP analysis using ab96328 binding POLR3A in 3T3 fibroblast whole cell lysate. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with undiluted primary antibody for 12 hours at 4°C. Protein binding was detected using real-time PCR. Positive control: tRNA le and tRNA ser. Negative Control:IgG.