Overview

  • Product name
  • Description
    Rabbit polyclonal to POLR3B
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1083-1133 of Human POLR3B (NP_060552.3).

  • Positive control
    • Human Breast Carcinoma, Hodgkins Lymphoma and Testicular Seminoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab114967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similarities
    Belongs to the RNA polymerase beta chain family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C128 antibody
    • DNA directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III subunit RPC2 antibody
    • FLJ10388 antibody
    • polr3b antibody
    • Polymerase (RNA) III (DNA directed) polypeptide B antibody
    • RNA polymerase III subunit C2 antibody
    • RNA polymerase III subunit RPC2 antibody
    • RPC2 antibody
    • RPC2_HUMAN antibody
    see all

Images

  • ab114967 at 1/250 dilution staining POLR3B in Formalin-fixed, Paraffin-embedded Human Hodgkin's Lymphoma tissue by Immunohistochemistry.
    Detection: DAB staining.
  • ab114967 at 1/500 dilution staining POLR3B in Formalin-fixed, Paraffin-embedded Human Breast Carcinoma tissue by Immunohistochemistry. Detection: DAB staining.

References

ab114967 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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