Overview

  • Product nameAnti-POLR3B antibody
    See all POLR3B primary antibodies
  • Description
    Rabbit polyclonal to POLR3B
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human POLR3B.

  • Positive control
    • Extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.
ELISA 1/20000.

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similaritiesBelongs to the RNA polymerase beta chain family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C128 antibody
    • DNA directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III subunit RPC2 antibody
    • FLJ10388 antibody
    • polr3b antibody
    • Polymerase (RNA) III (DNA directed) polypeptide B antibody
    • RNA polymerase III subunit C2 antibody
    • RNA polymerase III subunit RPC2 antibody
    • RPC2 antibody
    • RPC2_HUMAN antibody
    see all

Anti-POLR3B antibody images

  • All lanes : Anti-POLR3B antibody (ab75063) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 128 kDa
    Observed band size : >117 kDa (why is the actual band size different from the predicted?)

References for Anti-POLR3B antibody (ab75063)

ab75063 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"