Overview

  • Product nameAnti-POLR3B antibody
    See all POLR3B primary antibodies
  • Description
    Rabbit polyclonal to POLR3B
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    A region between residue 1083 and 1133 of human POLR3B using the numbering given in entry NP_060552.3.

  • Positive control
    • HeLa nuclear lysate 293T nuclear lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similaritiesBelongs to the RNA polymerase beta chain family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C128 antibody
    • DNA directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III 127.6 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit B antibody
    • DNA-directed RNA polymerase III subunit RPC2 antibody
    • FLJ10388 antibody
    • polr3b antibody
    • Polymerase (RNA) III (DNA directed) polypeptide B antibody
    • RNA polymerase III subunit C2 antibody
    • RNA polymerase III subunit RPC2 antibody
    • RPC2 antibody
    • RPC2_HUMAN antibody
    see all

Anti-POLR3B antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3B with ab86143 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-POLR3B antibody (ab86143) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 128 kDa
    Observed band size : 128 kDa


    Exposure time : 3 minutes
  • Detection of Human POLR3B by Immunoprecipitation, using ab86143 at 3µg/mg lysate. Image shows immunoprecipitated POLR3B detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upsatream eptiope in lane 1, ab86143 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-POLR3B antibody (ab86143)

ab86143 has not yet been referenced specifically in any publications.

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