Overview

  • Product name
  • Description
    Rabbit polyclonal to POLR3D
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 175 and 225 of human POLR3D (NP_001713.2).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab86786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 10 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    POLR3D is a DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • AI326118 antibody
    • AW489084 antibody
    • BN51 (BHK21) temperature sensitivity complementing antibody
    • BN51 antibody
    • BN51T antibody
    • DNA directed RNA polymerase III 47 kDa polypeptide antibody
    • DNA directed RNA polymerase III subunit D antibody
    • POLR3D antibody
    • Polymerase (RNA) III (DNA directed) polypeptide D antibody
    • Polymerase (RNA) III (DNA directed) polypeptide D, 44kDa antibody
    • Protein BN51 antibody
    • RNA polymerase III subunit C4 antibody
    • RPC4 antibody
    • RPC53 antibody
    • Temperature sensitive complementation cell cycle specific tsBN51 antibody
    • TSBN51 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3D with ab86786 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-POLR3D antibody (ab86786) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 3 minutes
  • Detection of POLR3D by Western Blot of Immunprecipitate.
    ab86786, at 1 µg/ml, staining POLR3D in HeLa whole cell lysate immunoprecipitated using ab86786 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab86786 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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