1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
POLR3D is a DNA dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling POLR3D with ab86786 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - POLR3D antibody (ab86786)
All lanes : Anti-POLR3D antibody (ab86786) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa Observed band size: 44 kDa
Exposure time: 3 minutes
Immunoprecipitation - POLR3D antibody (ab86786)
Detection of POLR3D by Western Blot of Immunprecipitate.
ab86786, at 1 µg/ml, staining POLR3D in HeLa whole cell lysate immunoprecipitated using ab86786 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.