Overview

  • Product nameAnti-POLR3K antibody
    See all POLR3K primary antibodies
  • Description
    Rabbit polyclonal to POLR3K
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 6-108 of Human POLR3K

  • Positive control
    • Human testis tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similaritiesBelongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11 antibody
    • C11 RNP3 antibody
    • DNA directed RNA polymerase III subunit K antibody
    • DNA directed RNA polymerase III subunit RPC10 antibody
    • DNA directed RNA polymerases III 12.5 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit K antibody
    • DNA-directed RNA polymerase III subunit RPC10 antibody
    • hRPC11 antibody
    • HsC11p antibody
    • My010 antibody
    • POLR3K antibody
    • Polymerase (RNA) III (DNA directed) polypeptide K antibody
    • Polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa antibody
    • RNA polymerase III 12.5 kDa subunit antibody
    • RNA polymerase III subunit (hRPC11) antibody
    • RNA polymerase III subunit C10 antibody
    • RNA polymerase III subunit C11 antibody
    • RNA polymerase III subunit CII antibody
    • RPC10 antibody
    • RPC10_HUMAN antibody
    • RPC11 antibody
    • RPC12.5 antibody
    see all

Anti-POLR3K antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab121238 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-POLR3K antibody (ab121238) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
  • Immunohistochemical staining of paraffin embedded Human testis tissue, using 1/50 ab121238, shows strong nuclear positivity in cells of seminiferus ducts.

References for Anti-POLR3K antibody (ab121238)

ab121238 has not yet been referenced specifically in any publications.

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