Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)

Overview

  • Product name
    Anti-Poly (ADP-Ribose) Polymer antibody
    See all Poly (ADP-Ribose) Polymer primary antibodies
  • Description
    Chicken polyclonal to Poly (ADP-Ribose) Polymer
  • Specificity
    This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Predicted to react with a wide range of species due to sequence homology.
  • Immunogen

    Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab14460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.
ELISA Use at an assay dependent dilution. It is recommended to start with a 1/100 dilution.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance
    Poly (ADP-Ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer poly (ADP-Ribose), with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. Poly (ADP-Ribose) polymer becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of poly (ADP-Ribose) polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
  • Alternative names
    • pADPr antibody
    • Poly ADP ribose antibody

Images

  • Poly (ADP-Ribose) Polymer staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.



  • Predicted band size : 1 kDa

    Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.

  • Poly (ADP-Ribose) Polymer staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml

  • ab14460 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Horton JK  et al. DNA polymerase ß-dependent cell survival independent of XRCC1 expression. DNA Repair (Amst) 26:23-9 (2015). Read more (PubMed: 25541391) »
  • Brand S  et al. Oxidative DNA damage in kidneys and heart of hypertensive mice is prevented by blocking angiotensin II and aldosterone receptors. PLoS One 9:e115715 (2014). Read more (PubMed: 25551569) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your message. I am very pleased to hear you would like to accept our offer and test ab14459 and ab14460 in IP.

ab14459
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Expiration date: 4th Feb 2013

ab14460
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Thank you for your message. i hope you enjoyed the EMBO conference.

I am sorry to confirm that to our knowledge, ab14459 and ab14460 have not been tested in IP. They have been tested in western blotting.

Therefore, I can offer a d...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (HeLa cells)
Permeabilization
Yes - 0.5% Triton X100
Specification
HeLa cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Alexander Rapp

Verified customer

Submitted Apr 18 2008

It is recommended to start at a 1:100 dilution. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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