Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.
Our Abpromise guarantee covers the use of ab14460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.|
|ELISA||Use at an assay dependent dilution. It is recommended to start with a 1/100 dilution.|
|IHC-P||Use at an assay dependent dilution.|
Poly (ADP-Ribose) Polymer staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.
Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.
Poly (ADP-Ribose) Polymer staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml
ab14460 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.